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LiScript 1st strand cDNA Synthesis Kit (+gDNA wiper) contains all components needed to synthesize high-quality 1st strand cDNA. Reaction products are applicable to subsequent PCR, qPCR and PCR cloning. The pre-treatement of 4× gDNA wiper Mix ensures RNA template completely free of residual genomic DNA, which ensures quantitative results more reliable. 2× RT Mix contains optimized buffer system and dNTP; Enzyme Mix contains LiScript Reverse Transcriptase and RNase inhibitor. Oligo (dT)18 and Random hexamers can be chosen as primers for reverse transcription as needed. Compare with Oligo dT18, Oligo dT23VN in Kit has stronger anchoring ability for Poly A+ RNA, which makes the reverse transcription efficiency is higher. Choose Oligo dT23VN accordingly, Random hexamers or gene specific primers for reverse transcription primer.
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